Question: You Would Like To Produce Large Amounts Of A Protein Encoded By The Gene Wmu1 In E. Coli Using A Specific Plasmid. A). Draw An Example Of A Plasmid That May Be Used In The Expression Of This Gene With All Required Elements For Expression In E. Coli. B). What Element(s) On The Plasmid Should You Optimize In Order To Improve Transcription Of Gene Wmu1? …

You would like to produce large amounts of a protein encoded bythe gene wmu1 in E. coli using a specific plasmid.

a). Draw an example of a plasmid that may be used in theexpression of this gene with all required elements for expressionin E. coli.

b). What element(s) on the plasmid should you optimize in orderto improve transcription of gene wmu1?

c). What element(s) on the plasmid should you optimize in orderto increase the replication of the plasmid in E. coli?