Question: You Decide That You Want To Further Investigate The CD4+ Population To Determine The Specific T, Cells Present. You Thaw The Same Mouse Spleen Single Cell Suspension Used Previously But This Time Perform Intracellular Cytokine Staining. The Antibody Cocktail Used For Staining The Cell Population Contained Antibodies Against The Following Cytokines: …

You decide that you want to further investigate the CD4+ population to determine the specific T, cells present. You thaw the same mouse spleen single cell suspension used previously but this time perform intracellular cytokine staining. The antibody cocktail used for staining the cell population contained antibodies against the following cytokines: IL-2, IL-4, IL-6, IL- 10, IL-17, and IFN-y. The cells were also stained with antibodies against surface markers CD3, CD4, and CD8 before performing the intracellular cytokine staining. Below is a dual parameter dot plot showing expression of CD4 and IFN-y. 04 A6 47 IFN gamma FITC 5. Based on the above dot plot, which T cell subtype is present? Justify your answer. (2pts) 6. Based on the above dot plot, what cell type could be present in the lower right quadrant? Justify your answer. (2pts) 7. What is another way to confirm the T cell subtype besides identifying the cytokine profile? Briefly explain your answer. (2pts)