Question: The URA Pone Is Required To Make Walinya. Apenaticist Obtained A Yeast Strain Hom Another Lab For An Experiment And Decovered La Artrophic For Acil Due To A Mutation In The Uragana. She Sequenced The Band Urales And Discovered A Single Base Par Substitution In The Ura3 Aleshown Below) URA … MATOTT… μη2 5’… AATOTA,’ 1.TTACALCCT…5 ..TTACCACCI …

The URA pone is required to make walinya. Apenaticist obtained a yeast strain hom another lab for an experiment and decovered la artrophic for acil due to a mutation in the uragana. She sequenced the Band urales and discovered a single base par substitution in the ura3 aleshown below) URA … MATOTT… μη2 5’… AATOTA,’ 1.TTACALCCT…5 ..TTACCACCI To was the strain, the needs to be protrophic for also she decided to revert the rulele back to URA? To ne treguney of reson she benede una) celo whataper, and then pland the mutagene culs onto medium lacking rao 10 select for colonies that could grow Assume that only one base par substitution occurs in each revitant The magen sharad on and were Type of mutagen Mode of action hydroxylamine Modifes Csopars WA me bendras Moes Asians with Od Modes so that is with Bestilo mais belor TRUE Themen in ons Themen MOST LELUAS dronne Theme OST LIKELYURT me OST LIKELTURA The WOTE GURATION 10 The URAI is required to make uracil in yeast Agencies inedia yeast an from another lab for an experiment and discovered to strophic for ao dosto a mutation in the user the sequenced the URAJ and um 3 alleles and discovered a single base par substitution in the ura3 allele shown below) URAS MAIG TOGA. – was.. MATUOTOA… 3 TTACAROCE.. 3… TACCCCT… To use the strain, she needs to be protrophic for urci so she decided to revert the oralele back to URA) To increase the foundersions and serves with a mutagen, and then plated the mageund berils onto medium backing to select for colonies that could grow Assuna that only one base par un achetant. The mutagen she had on and we Type of mutagen Mode of action hydroxylamine Modities Csomatitas XY makes douradores Nous acid Modifies so that pairs with Modifies so that it pairs with Based on this information select the statements below that TRUE for at art for answer The mutton is transversion base substitution The mutagen MOST LIKELY to rower the 3 mutant to URAI is hydrome Tom MOST LIKELY mutant URA nous sod The rurager MOST LIKELY to revent the 3 mua URAT is the liter The mutationes transion base batution Theme MOST LIKELY to even the mutant URA E Xay QUESTION The URL be gardenyes A praticant tuned ayant en from another two turn experiment and discovered to otrophic for urmat de to a mutation in the wagen She need the URAT and ws and dongle below URAS WATOTO TOOT. TTACLECT ITALI.. To the transitors to the decided to the week LIRA Tomorena the version, thered the rol with a magen, and a plane temaened making to let forcat could yow Artur only one talebi Schevertant. The magans she had on and were Deltage Mode bydyn IM La MA Martes Ha Ha va la la gue THUÊ The MOSTEKELYURT The MOSTLY RAS Themen VOSTURE eURA The The MOSTOKELYURT The US மாரலாமா கம் கணe TA TINCT the whe TU VOTRE UAB The MOSTLY GULATION The URAJ generis equired to make uradil in yeast Agenciebtained a year trom another lab for an experiment and discovered the trophic foluto a mining the wels and discovered a single base parton in the sale shown below URAL …ATOTTOGA… wa ‘…AATOGEOGR… TTACAMOGT.. …TTACCACCT… To use the strain, she needs to be protrophie forurile she decided to rever the wall back to URAI To increase the one of wine treated the races with a demo cells on medium lacking in to select for Golonies that could grow. Assume that only one base par son cours in each vertant The mutagens she had on hand Type of mutagen Mode of action Modes pars with Nous sod Ohis Modifs As atkan Moses so that Based on this select the statement below the TRUE for me pot for an income awe The mutation is is a travessuto The mutagen MOST LIKELY to revert the autant URAT is hydroxyanin The mutagen MOST UKELY to mano URAIS The mutagen MOST UKELY never the matte URAT the The union in was transion base The magen MOST LIKELY to revert the autoURATEX-rays The URA3 gene is required to make uracil in yeast. A geneticist obtained a yeast strain from another lab for an experiment and discovered it is auxotrophic for uracil due to a mutation in the ura3 gene. She sequenced the URA3 and ura3 alleles and discovered a single base pair substitution in the ura3 allele (shown below). URA3: 5’…AATGTTGGA…3 ura3:5’…AATGGTGGA…31 3′ TTACAACCT…5’ 31… TTACCACCT…5 To use the strain, she needs it to be protorophic for uracil so she decided to revert the ura3 allele back to URA3. To increase the frequency of reversion, she treated the ura3 cells with a mutagen, and then plated the mutagenized cells onto medium lacking uracil to select for colonies that could grow. Assume that only one base pair substitution occurs in each revertant. The mutagens she had on hand were: Type of mutagen Mode of action hydroxylamine Modifies C so that it pairs With A X-rays makes double strand breaks Nitrous acid Modifies A so that it pairs with C Oxidizer Modifies G so that it pairs with A Based on this ormation select the statements below that are TRUE 2 points for a corect answer 1 point for an incorrect answer Pegel CISCUITS that could grow. Assume that only one base pair substitution occurs in each revertant. The mutagens she had on hand Type of mutagen Mode of action hydroxylamine Modifies so that it pairs with A X-rays makes double strand breaks Nitrous acid Modifies A so that it pairs with C Oxidizer Modifies G so that it pairs with A Based on this information select the statements below that are TRUE 2 points for a correct answer. -1 point for an incorrect answer. WA The mutation in ura3 is a transversion base substitution. 28 The mutagen MOST LIKELY to revert the ura3 mutant to URA3 is hydroxytamine. The mutagen MOST LIKELY to revert the ura3 mutant to URA3 is nitrous acid. The mutagen MOST LIKELY to revert the ura3 mutant to URA3 is the oxidizer. E The mutation in ura3 is a transition base substitution, The mutagen MOST LIKELY to revert the ura3 mutant to URA3 are X-rays