Question: The LocZ Gene Codes For The Beta Galactosidase Enzyme That Is Capable Of Processing Sugar Substrates Into A Blue Product, Turning The Entire Bacterial Cell Blue. Imagine You Are Attempting To Create A Recombinant Plasmid According To The Plan In The Figure (see Figure 1 In The Exam 3 Figures Document). What Conclusion Would You Draw From A Blue Bacterial …
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The LocZ gene codes for the beta galactosidase enzyme that is capable of processing sugar substrates into a blue product, turning the entire bacterial cell blue. Imagine you are attempting to create a recombinant plasmid according to the plan in the figure (see figure 1 in the exam 3 figures document). What conclusion would you draw from a blue bacterial cell? KEY lacZ gene Restriction site O amp gene Origin of replication (ori) OOO A. bacteria failed to take up the recombinant plasmid B. an intact Lacz gene is being expressed the gene of interest was hot inserted at the target recombinant site D.B and C – an intact Lacz gene is being expressed AND the gene of interest was not inserted at the target recombinant site E. A and B and C-bacteria failed to take up the recombinant plasmid AND an intact Lacz gene is being expressed AND the gene of interest was not inserted at the target recombinant site
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