Question: The Fractionation Of A Sample From Rat Liver . The Experiment Summarized As: A Subcellular Fraction Was Obtained By Differential Centrifugation From Rat Liver. The Sample, Containing Fragmented Plasma Membrane And Endoplasmic Reticulum In The Form Of Small Vesicles, Was Subjected To Equilibrium Density-gradient Centrifugation In A Sucrose Gradient. …

the fractionation of a sample from rat liver . theexperiment summarized as:

A subcellular fraction was obtained by differentialcentrifugation from rat liver. The sample, containing
fragmented plasma membrane and endoplasmic reticulum in the form ofsmall vesicles, was subjected to
equilibrium density-gradient centrifugation in a sucrose gradient.Fractions were collected and their
densities and phospholipid, protein, and RNA contents weredetermined (Figure A). Fractions of 1.130 (a),
1.175 (b), 1.204 (c) and 1.238 g/cm3
(d) were further processed and visualized by electronmicroscopy
(Figure B).

a) provide the differential centrifugation and equilibrium densitygradient centrifugation of these three characteristics: 1.usefulness 2. principle of method 3. limitation.
b) Use your knowledge of electron microscopy to explain in your ownwords how the fractions were
“further processed and visualized by electron microscopy”. Youranswer should include sample
preparation and the details of how the image is formed .
please answer these both parts a and b in detail