Question: Task 1: Mutation In The Middle! 1 Last Week, You Should Have Identified Three Recessive, X-linked Loci. If You’re Not Sure You’ve Identified The Correct Loci As X-linked Recessive, Please Check Your Reasoning With Your Classmates, Bethany, Or Dr. Bastiaans 1. Your First Job Is To Generate The Flies You Will Need For A Three-point Cross Involving Loci …

Task 1: Mutation in the middle! 1 Last week, you should have identified three recessive, X-linked loci. If you’re not sure you’ve identified the correct loci as X-linked recessive, please check your reasoning with your classmates, Bethany, or Dr. Bastiaans 1. Your first job is to generate the flies you will need for a three-point cross involving loci on the X chromosome. One of these flies should be a female heterozygous for all three mutant alleles. You should be confident all three of her mutant alleles are on the same chromosome and all three of her wild type alleles are on the same chromosome. Describe how you will generate a female fly with these characteristics (2 pts.). 2. Once you have the fly in question 1, you will need to cross her to another fly to carry out the 3-point cross. Obviously, this will be a male fly, but what genotype should he have at the three loci we are following? Explain why (1 pt.). 3. Carry out the mating between the female you described in question 1 and the male you described in question 2. Which of the three loci is in the middle of the other two? Explain how you can tell from the results of your cross (2 pts.). Task 2: Drumroll please…the three-point cross! 1. Define the symbols you will use for mutant and wild type alleles at the three X-linked loci you identified. Then, fill in the table below with the gamete categories, F2 phenotypes, and numbers of offspring in each category that resulted from your three-point cross. You will receive 2 points for correctly filling in the gametes, F2 phenotypes, and numbers of flies observed that resulted from each kind of crossover event (8 pts. total). Gametes Gamete origin Non-crossover 712 phenotypes # of flies observed Single crossover (category 1) Single crossover (category 2) Double crossover 2. Based on the information in the table from question 1, calculate the map distance between each pair of loci. Show your work (2 pts.). Correct answers with no work shown will receive zero credit, but incorrect answers with work shown may receive partial credit.