Question: Step 2: Gather Absorbance Data From Your Dilutions On The Spectrophotometer. All Of These Measurements Were Gathered At 600nm Using The Same Cuvette. Here Are The Absorbance Measurements We Gathered For The Protein Standard Curve That We Conducted In Triplicate (Trial 1-5). Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Stock 0.651 0.712 0.632 0.661 0.684 …
Step 2: Gather absorbance data from your dilutions on thespectrophotometer. All of these measurements were gathered at 600nmusing the same cuvette.
Here are the absorbance measurements we gathered for the proteinstandard curve that we conducted in triplicate(Trial 1-5).
| Trial 1 | Trial 2 | Trial 3 | Trial 4 | Trial 5 | |
| Stock | 0.651 | 0.712 | 0.632 | 0.661 | 0.684 |
| D1 | 0.121 | 0.118 | 0.126 | 0.134 | 0.119 |
| D2 | 0.024 | 0.031 | 0.048 | 0.037 | 0.038 |
| D3 | 0.012 | 0.016 | 0.021 | 0.027 | 0.019 |
| D4 | 0.004 | 0.008 | 0.006 | 0.01 | 0.007 |
| D5 | 0.001 | 0.006 | 0.005 | 0.007 | 0.004 |
Step 3: Create the standard curve graph.
Now, we need to use this data to create a graph of the standardcurve using the concentration values you calculated from the serialdilution and the measured absorbance values above.
Before moving forward, record these data in your notes -identifying the absorbance values for each of the different proteinconcentrations in the serial dilution that you calculated in theprevious step.
Is this your assignment or some part of it?
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