Question: Regulation Of Gene Expression In Bacteria Involve An Operon. The Operon Consists Of All Of The Following Components Except For … A. A Promoter. A Primer. C. An Operator. D. A Transcription Unit. B. What Is The Most Logical Sequence Of Steps For Splicing Foreign DNA Into A Plasmid And Then Inserting The Plasmid Into A Bacterial Cell? 1. Transform Bacteria …

Regulation of gene expression in bacteria involve an operon. The operon consists of all of the following components except for … a. a promoter. a primer. c. an operator. d. a transcription unit. b. What is the most logical sequence of steps for splicing foreign DNA into a plasmid and then inserting the plasmid into a bacterial cell? 1. Transform bacteria with a recombinant DNA molecule. II. Cut the plasmid with a restriction enzyme. III. Extract the plasmid from a bacterial cell. IV. Insert foreign DNA into a plasmid with matching “sticky ends” produced by the same restriction enzyme. V. Use ligase to splice foreign DNA to plasmid DNA. a. steps I, II, IV, III, V in that order b. steps II, III, IV, V, I in that order C. steps III, II, IV, V, I in that order TUT d. formation of mRNA.