Question: III. Using Tonicity To Predict Effects On Cells If You Know The Tonicity Of A Solution You Can Predict Its Effect On Cells. If You Put RBCs Into En Isotonic Solution, The Nonpenetrating Solute Concentrations On Both Sides Of The Membrane Are The Same. (For The Purpose Of These Examples, We Will Assume That The Osmolarity Of The Solutions Is Based On …
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III. Using tonicity to predict effects on cells If you know the tonicity of a solution you can predict its effect on cells. If you put RBCs into en isotonic solution, the nonpenetrating solute concentrations on both sides of the membrane are the same. (For the purpose of these examples, we will assume that the osmolarity of the solutions is based on nonpenetrating solute concentrations. That is, isosmostic = isotonic; byposmotic = hypotonic, hyperosmotic = hypertonic) ECF = 300 mOsm ICF = 300 mOsm Will osmosis occur? In which direction? Will the cell volume increase, decrease, or stay the same size If you put RBCs into a hypotonic solution, the nonpenetrating solute concentration on the outside of the cell is lower than on the inside. ECF = 250 mOsm Will osmosis occur? In which direction? Will the cell volume increase, decrease, or stay the same size? ICF = 300 mOsm When RBCs swell up their membrane becomes very leaky, and the hemoglobin leaks out, leaving an empty plasma membrane (ghost). This phenomenon is called hemolysis (from hemo – blood and lysis – disintegration) even though the cell does not actually rupture. You just cannot see the cells anymore because there is nothing left inside them. The hemoglobin is evenly distributed in the solution instead of being inside the PEC member Focus h (United States) If you put RBCs into a hypotonic solution, the nonpenetrating solute concentration on the outside of the cell is lower than on the inside ECF = 250 mOsm Will osmosis occur In which direction? Will the cell volume increaso, decrease, or stay the same sizo? ICF = 300 mm When RBCs swell up their membrane becomes very leaky, and the hemoglobin leaks out, leaving an empty plasma membrane (ghost). This phenomenon is called hemolysis (from bemo-blood and lysis – disintegration) even though the cell does not actually rupture. You just cannot see the cells anymore because there is nothing left inside them. The hemoglobin is evenly distributed in the solution instead of being inside the RBC membranes. ECF = 350 mm Will osmosis occur? in which direction? ICF = 300 mm Will the cell volume increaso, decrease or stay the same size? Page 4 of 10
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