Question: Consider The Deep-sea Hydrothermal Vent Worm Collagen. This Collagen, Rather Than 4- Hydroxyproline In The Y Position Of The Gly-X-Y Repeat Found In Collagen, The Y Position Is Replaced With Thr, And The Threonine Is O-glycosylated. Studies With Peptides Having The Sequence (G-P-T) Repeated In Sequence 10 Times (GPT)10 Has No Collagen Structure, Wherease …

Consider the deep-sea hydrothermal vent worm collagen. This collagen, rather than 4- hydroxyproline in the Y position of the Gly-X-Y repeat found in collagen, the Y position is replaced with Thr, and the threonine is O-glycosylated. Studies with peptides having the sequence (G-P-T) repeated in sequence 10 times (GPT)10 has no collagen structure, wherease the same peptide with the Thr O-glycosylated with galactose exhibits a collagen structure with a melting terr:perature of 50 degrees. Given the structure of collagen contains three chains and each is in a polyproline Il helix, how might glycosylation affect the formation of this structure? (Hint: consider the glycosaminoglycans).